Per the order of the Ltd "Izumrud" placed within the period of 20.10.2005 to 28.02.2006 on the agreement N31 dated 20.10.2005 there were investigations undertaken to research the disinfecting influence of the solution AQUAECA (anolyte) on the virus infection H5N3 and on the chicken birth (hatching) in the case of coating the egg shell with the solution and injecting this into the chicken embryo (REC).

  For undertaking the research there have been presented 4 sets of AQUAECA (anolyte): the 1^st set -2 examinations, the 2^nd set -2 examinations, the 3^rd set - 4 examinations, the 4^th set - 2 examinations.

  AQUAECA (anolyte) was produced: the 1st set in September 2005, 2^th set - in October 2005, the 3^th set - in January 2006, the 4^th set - in January 2006. In the represented sets the concentration of hydrogen ions pH was from the concentration of the active chlorine C.ax - from mg/l.

  The research has used the virus of the bird flu of the fifth serum type (H5N3) - 4 sets with glue activity from 1:128 to 1: 256; infection activity ALD 50/ml from 10^-8,45 to 10^-8,75.

  The developing chicken embryos of the 10-day period of incubation have been used for the research.

  On the basis of the research, the following conclusions have been made:

  1. AQUAECA (anolyte) does not play an inhibiting influence on the birth (hatching) of the chickens, both in the case of coating the egg shell and injecting this into the embryo in amount of 0,2 ml. The born chickens were capable of living.

  AQUAECA (anolyte) used for coating the egg shell and injecting into the embryo had pH from the concentration of the active chlorine was mgr/liter.

  2. AQUAECA (anolyte) has the coagulating influence on the virus containing extra embryo liquid (kills the virus activity)

  The control of the virus presence in the coagulated extra embryo liquid was undertaken through injecting the 0,2 ml of coagulated extra embryo liquid with the further incubation during the 96 hours with the temperature 37 ± 0.5 C. After the incubation the investigation on the presence of the living virus has been undertaken. The virus in the coagulated extra embryo liquid was not found.

  On the basis of the information above, the conclusion has been made that the disinfecting solution AQUAECA (anolyte) kills the virus of the bird flu and is safe for the chicken embryo, chickens and adult poultry.

  Director of the State Research Institution The All-Russia Scientific Research Vet Institute of Poultry Breeding Doctor of Veterinary science A.D.Dzhavadov

  Executor The senior research lab assistant of the virus department and the department of the safe production A.M. Amdiy A.M. Amdiy